Austin FC II
Atlanta United 2
Carolina Core FC
Chattanooga FC
Chicago Fire FC II
Colorado Rapids 2
Columbus Crew 2
Crown Legacy FC
Connecticut United FC
FC Cincinnati 2
Hong Kong Team
Houston Dynamo 2
Huntsville City FC
Inter Miami CF II
Los Angeles Football Club 2
North Texas SC
NYCFC II
Orlando City B
Real Monarchs
Red Bull New York II
New England Revolution II
Rochester New York FC
Sporting KC II
St Louis CITY2
Tacoma Defiance
Timbers2
The Town FC
Philadelphia Union II
Ventura County FC
Whitecaps FC 2