Austin FC II
Atlanta United 2
Carolina Core FC
Chattanooga FC
Chicago Fire FC II
Colorado Rapids 2
Columbus Crew 2
Crown Legacy FC
FC Cincinnati 2
Hong Kong Team
Houston Dynamo 2
Huntsville City FC
Inter Miami CF II
Los Angeles Football Club 2
New York Red Bulls II
North Texas SC
NYCFC II
Orlando City B
Real Monarchs
New England Revolution II
Rochester New York FC
Sporting KC II
St Louis CITY2
Tacoma Defiance
Timbers2
The Town FC
Philadelphia Union II
Ventura County FC
Whitecaps FC 2